Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols

Description: Alzheimer's disease and frontotemporal dementia, two of the most prevalent neurodegenerative diseases, are dreaded disorders that attack the neural networks underlying memory and personality, systems that make us who we are. Major breakthroughs in recent years have spurred new research approaches to these conditions. In Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols, expert investigators bring together the many divergent areas of expertise used to study these diseases, including behavior, electrophysiology, confocal microscopy, and hardcore biochemistry. Beginning with an overview of the two diseases and contemporary research on them, the book continues with specialized protocols for working with amyloid-ß peptide, tau, and apolipoprotein E as well as experimental systems for studying AD and FTD, including cell and animal models, and outcome measures that can be used to assess neuronal function in these systems. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols serves as an ideal guide for all scientists pursuing the cause and cure of these widespread and nightmarishly debilitating disorders. 1. Contemporary Approaches to Alzheimer's Disease and Frontotemporal Dementia Erik D. Roberson Part I: Working with AD- and FTD-Related Molecules 2. Preparing Synthetic A in Different Aggregation States W. Blaine Stine, Lisa Jungbauer, Chunjiang Yu, and Mary Jo LaDu 3. Isolation of Low-n Amyloid ß-Protein Oligomers from Cultured Cells, CSF, and Brain Ganesh M. Shankar, Alfred T. Welzel, Jessica M. McDonald, Dennis J. Selkoe, and Dominic M. Walsh 4. Detecting A*56 Oligomers in Brain Tissues Mathew A. Sherman and Sylvain E. Lesné 5. Assessing A Aggregation State by Atomic Force Microscopy Justin Legleiter 6. Measuring APP Carboxy-Terminal Fragments Luke A. Esposito 7. Detection of APP Intracellular Domain (AICD) in Brain Tissue Sanjay W. Pimplikar and Anupama Suryanarayana 8. Cell-Based Assays for Regulators of Tau Biology Umesh K. Jinwal and Chad A. Dickey 9. Split GFP Complementation Assay for Quantitative Measurement of Tau Aggregation In situ Wanjoo Chun, Geoffrey S. Waldo, and Gail V.W. Johnson 10. Apolipoprotein E Expression and Purification Yvonne Newhouse and Karl H. Weisgraber Part II: Model Systems and Outcome Measures 11. Aß Toxicity in Primary Cultured Neurons Adriana Ferreira, Roxana C. Sinjoanu, Alexandra Nicholson, and Sara Kleinschmidt 12. Manipulation of Gene Expression in the Central Nervous System with Lentiviral Vectors Binggui Sun and Li Gan 13. Selecting a Mouse Model of Alzheimer's Disease Jeannie Chin 14. Monitoring Spatial Learning and Memory in Alzheimer's Disease Mouse Models Using the Morris Water Maze Kimberly Scearce-Levie 15. Step-by-Step In Situ Hybridization Method for Localizing Gene Expression Changes in the Brain Jorge J. Palop, Erik D. Roberson, and Inma Cobos 16. Real-Time Visualization of Axonal Transport in Neurons Yasuko Osakada and Bianxiao Cui 17. Quantifying Biomarkers of Cognitive Dysfunction and Neuronal Network Hyperexcitability in Mouse Models of Alzheimer's Disease: Depletion of Calcium-Dependent Proteins and Inhibitory Hippocampal Remodeling Jorge J. Palop, Lennart Mucke, and Erik D. Roberson 18. Epigenetic Changes in the Brain: Measuring Global Histone Modifications Gavin Rumbaugh and Courtney A. Miller

Price: 117 GBP

Location: Gloucester

End Time: 2025-01-13T05:41:31.000Z

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