Ubiquitin-Proteasome Protocols by Cam Patterson (English) Hardcover Book

Description: Ubiquitin-Proteasome Protocols by Cam Patterson, Douglas M. Cyr State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This volume is provides the tools for investigators to learn how to manipulate the ubiqutin-proteasome system to address questions in their own areas of reaserch. This borad field is covered in detail from basic biochemistry to cellular assays to discovery techniques using mass spectroscopic analysis. It will serve as a useful tool to accelerate discovery and enhance productivity. Back Cover The targeted addition of ubiquitin to proteins is now known to regulate many cellular events, with the enzymes involved in protein ubiquitination linked to a variety of clinical problems, such as cancer, heart disease, and immune dysfunction. In Ubiquitin-Proteasome Protocols, hands-on leaders in the field, many of whom originally developed the methods they describe, detail cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome- dependent functions. The protocols follow the successful Methods in Molecular Biology(tm) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes. Table of Contents Biochemical Methods to Study the Ubiquitin-Proteasome System.- Small-Molecule Inhibitors of Proteasome Activity.- Purification of E1 and E1-Like Enzymes.- Assays for RING Family Ubiquitin Ligases.- Ubiquitin Chain Synthesis.- Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast.- Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome.- Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation.- Assays of Proteasome-Dependent Cleavage Products.- Identification of Components of Protein Complexes.- Mass Spectrometric Determination of Protein Ubiquitination.- Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol.- Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation.- Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization.- Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions.- Measuring Ubiquitin Conjugation in Cells.- Assays for Proteasome Assembly and Maturation.- N-Terminal Ubiquitination.- Quantitating Defective Ribosome Products.- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation.- Endoplasmic Reticulum-Associated Protein Quality Control and Degradation.- Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells.- Aggresome Formation.- Detection of Sumoylated Proteins.- Proteasome Inhibitors in Cancer Therapy.- Parkinsons Disease. Review "...clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field." - Doodys Health Science Book Review Journal Long Description A collection of cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome-dependent functions. State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes. Review Text "...clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field." - Doodys Health Science Book Review Journal Review Quote "...clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field." - Doodys Health Science Book Review Journal Feature Includes supplementary material: sn.pub/extras Description for Bookstore A fresh take on the interwar years which challenges a number of conventional assumptions about the period, including: the navete of British appeasement; the futility of the League of Nations; the irrationality of German and Japanese expansionism; and the inadvertence of the American entry into war against Japan. Details ISBN1588292525 Author Douglas M. Cyr Language English ISBN-10 1588292525 ISBN-13 9781588292520 Media Book Format Hardcover DEWEY 572.76 Series Number 301 Year 2005 Place of Publication Totowa, NJ Country of Publication United States Edited by Cam Patterson Imprint Humana Press Inc. Short Title UBIQUITIN-PROTEASOME PROTOCOLS Residence Chapel Hill, NC, US Affiliation Chief, Division of Cardiology, Ernest and Hazel Craige Distinguished P Pages 381 Illustrations 172 Illustrations, black and white; XII, 381 p. 172 illus. DOI 10.1604/9781588292520;10.1385/1592598951 AU Release Date 2005-04-01 NZ Release Date 2005-04-01 US Release Date 2005-04-01 UK Release Date 2005-04-01 Publisher Humana Press Inc. Edition Description 2005 ed. Series Methods in Molecular Biology Edition 2005th Publication Date 2005-04-01 Alternative 9781617374531 Audience Professional & Vocational We've got this At The Nile, if you're looking for it, we've got it. 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